Detailed view for EHI_126160

Basic information

TDR Targets ID: 803438
Entamoeba histolytica, DNA excision repair protein, putative
Source Database / ID:  AmoebaDB  

pI: 9.9599 | Length (AA): 238 | MW (Da): 27884 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03834   Binding domain of DNA repair protein Ercc1 (rad10/Swi10)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005634   nucleus  
GO:0004519   endonuclease activity  
GO:0003684   damaged DNA binding  
GO:0006281   DNA repair  

Metabolic Pathways

Nucleotide excision repair (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
14 210 4bxo (B) 18 214 17.00 0.000000042 0.66 1.02933 -0.25
26 210 2bgw (A) 31 227 18.00 0 1 0.978511 -0.14
137 210 2a1j (B) 221 292 22.00 0 0.71 0.734124 -1.88
147 212 1x2i (A) 3 68 23.00 0 0.99 0.751511 -1.73

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Rahman HM-1 IMSS Trophozoite. Hon CC
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_128697)

Species Accession Gene Product
Arabidopsis thaliana AT3G05210   DNA excision repair protein ERCC-1
Babesia bovis BBOV_III011710   hypothetical protein
Brugia malayi Bm1_36110   Helix-hairpin-helix motif family protein
Candida albicans CaO19.2469   similar to ERCC1, known complex with ERCC4 protein
Candida albicans CaO19.10006   similar to ERCC1, known complex with ERCC4 protein
Caenorhabditis elegans CELE_F10G8.7   Protein ERCC-1
Cryptosporidium hominis Chro.60302   excision repair cross-complementing rodent repair deficiency,complementation group 1
Cryptosporidium parvum cgd6_2610   ERCC1 excision repair 1; C-terminal HhH domain
Dictyostelium discoideum DDB_G0267682   DNA excision repair protein 1
Drosophila melanogaster Dmel_CG10215   CG10215 gene product from transcript CG10215-RA
Echinococcus granulosus EgrG_000614500   DNA excision repair protein ERCC 1
Entamoeba histolytica EHI_126160   DNA excision repair protein, putative
Echinococcus multilocularis EmuJ_000614500   DNA excision repair protein ERCC 1
Homo sapiens ENSG00000012061   excision repair cross-complementation group 1
Loa Loa (eye worm) LOAG_05710   helix-hairpin-helix domain-containing protein family protein
Mus musculus ENSMUSG00000003549   excision repair cross-complementing rodent repair deficiency, complementation group 1
Neospora caninum NCLIV_065530   DNA repair protein rad10 domain-containing protein, putative
Oryza sativa 4349131   Os10g0518900
Plasmodium berghei PBANKA_0301200   ERCC1 nucleotide excision repair protein, putative
Plasmodium falciparum PF3D7_0203300   ERCC1 nucleotide excision repair protein, putative
Plasmodium knowlesi PKNH_0417800   ERCC1 nucleotide excision repair protein, putative
Plasmodium vivax PVX_003600   ERCC1 nucleotide excision repair protein, putative
Plasmodium yoelii PY06905   DNA repair protein rad10, putative
Saccharomyces cerevisiae YML095C   Rad10p
Schistosoma japonicum Sjp_0202620   DNA excision repair protein ERCC-1, putative
Schistosoma mansoni Smp_022470   excision repair cross-complementing 1 ercc1
Toxoplasma gondii TGME49_249330   DNA repair protein rad10 subfamily protein
Theileria parva TP01_0997   hypothetical protein
Trichomonas vaginalis TVAG_093320   excision repair cross-complementing 1 ercc1, putative

Essentiality

EHI_126160 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
TGME49_249330 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens excision repair cross-complementation group 1 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0053 1 1
0.0115 1 1
0.0079093 1 1
0.0478 0.736 1
0.0519 0.3698 1
0.0065 1 1
0.0065 1 1
0.0412 1 1
0.0057 1 1
0.0052 1 1
0.0065 1 1
0.006 1 1
0.0065 1 1
0.0065 1 1
0.0065 1 1
0.0418 1 1
0.0416 1 1
0.0225 0.4545 1
0.0065 1 1
0.0259 0.6229 1
0.0065 1 1
0.0065 1 1
0.042 1 1
0.0065 1 1
0.0065 1 1
0.0065 1 1
0.0065 1 1
0.00766052 0.909366 0.909366
0.0065 1 1
0.00723308 1 1
0.0065 1 1
0.0052 1 1
0.0065 1 1
0.0065 1 1
0.0056 1 1
0.0065 1 1
0.00777865 1 1
0.0065 1 1
0.0065 1 1
0.0065 1 1
0.0065 1 1
0.0065 1 1
0.0162 1 1
0.0065 1 1
0.0065 1 1
0.0065 1 1
0.0216 1 1
0.0065 1 1
0.0479 0.3031 1
0.0065 1 1
0.0065 1 1
0.0065 1 1
0.0065 1 1
0.0065 0.463 1
0.0519 0.3698 1
0.006 1 1
0.0389 1 1
0.0065 1 1
0.0065 1 1
0.0162 1 1
0.0055 1 1
0.022 0.3341 1
0.0065 1 1
0.0057 1 1
0.0065 1 1
0.0065 1 1
0.0065 1 1
0.0065 1 1
0.0065 1 1
0.0343 0.7497 1
0.0116 0.3833 0.8841
0.0065 1 1
0.0053 1 1
0.0276 0.3326 1
0.0052 1 1
0.0053 1 1
0.0116 0.3833 0.8841
0.0065 1 1
0.0065 1 1
0.0065 1 1
0.0116 0.3833 0.8841
0.0065 1 1
0.0479 0.3031 1
0.0065 1 1
0.0065 1 1
0.0276 0.279 1
0.0065 1 1
0.0065 1 1
0.0065 1 1
0.0065 1 1
0.0054 1 1
0.0065 1 1
0.0065 1 1
0.0478 0.736 1
0.0058 1 1
0.0065 1 1
0.0065 1 1
0.0065 1 1
0.0065 1 1
0.0244 0.5643 1
0.0052 1 1
0.0055 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier EHI_126160 (Entamoeba histolytica), DNA excision repair protein, putative
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